FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054291
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG21765310.595042279793857No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC1181245.750110378714603No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG393671.916330257008379No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG238661.1617633529037512No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA215151.047319975602288No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG194830.9484050701677611No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC156130.7600189067663734No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG123480.6010832934574508No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC101880.49593752783807166No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA90550.44078467948309175No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC76630.3730240749728252No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA57350.2791717434384905No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA56770.27634838491722935No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA46460.22616075327205348No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGC36520.17777422964906142No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT34030.1656532594457163No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA33980.1654098664697455No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC32200.15674507652518557No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC31020.15100100229227506No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC30210.14705803608154833No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT29630.1442346775602872No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG29380.1430177126804333No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC26660.1297771347876226No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT26350.12826809833660371No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23720.11546562780054043No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC23320.11351848399277416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23250.11317773382641505No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTC21420.10426955090588431No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)