FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481461
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA1224228.263599244259552No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC958616.470706957523688No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG368362.4864643753699895No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA168131.1348931898983503No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA140970.9515606553260598No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG134390.9071450412801958No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG107180.7234750020419033No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC106070.7159823984566587No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC76290.514964619385863No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT71700.4839816910468787No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC58280.39339543869193994No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT36490.24631090524826504No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA34650.23389073353939116No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA33320.2249131094237378No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC31770.21445046477767557No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT27900.18832760362912018No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA24390.16463477607577925No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGG24260.16375726394417403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22920.15471213889532023No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.14667952784447244No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC19810.13371934866999535No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG19390.1308843094755785No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC18760.12663175068395321No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT18590.12548423481954638No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT18310.12359420868993513No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC17900.12082667042871867No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA17700.11947665176471065No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC16580.11191654724626568No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA15150.10226391379860827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)