FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204763
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC625105.188572358214852No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC484064.0178856754399No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA147271.2223980982151676No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC68350.5673315000543675No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA63120.5239204723252624No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG59930.49744223552682143No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC54230.4501300255734945No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA42930.3563356444379517No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC37290.3095214577472914No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT29450.24444641809218906No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC27800.2307507783688576No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT23540.19539112671952907No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA22100.1834385684155307No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT18000.14940697879997974No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT17820.14791290901197995No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA16790.13936350966953667No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT15530.12890502115353808No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC14820.12301174587864999No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA14730.1222647109846501No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14490.1202726179339837No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC13410.1113081992059849No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13180.10939911003242961No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA13000.10790504024442982No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC12940.10740701698176323No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC12910.10715800535042992No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCA12340.10242678435509722No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)