FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543410
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC1524499.877414296913976No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC1457659.444347257047706No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA372252.411867229057736No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG247551.603916004172579No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA231911.50258194517335No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC198471.2859188420445637No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA125470.8129401779177277No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC83940.5438606721480358No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG72010.4665642959421022No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC59850.38777771298617997No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA53880.34909712908430035No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC45660.2958384356716621No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG40060.25955514088932946No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA39210.25404785507415395No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT34510.22359580409612478No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG30460.19735520697675926No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG29080.1884139664768273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.17707543685734833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26510.17176252583564963No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC23160.15005734056407566No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA22890.1483079674227846No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT22390.14506838753150492No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC22340.14474442954237693No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA22220.14396693036846983No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA21620.14007943449893417No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC18740.12141945432516313No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC17300.11208946423827758No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC16660.10794280197743955No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)