FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791890
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC264043.334301481266338No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG91091.1502860245741202No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG65650.8290292843703039No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT64730.817411509174254No Hit
GTGTATAAGAGACAGCCAGGTCTGAAGGCAGTCTCTTATACACATCTCCG45740.5776054755079619No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA44700.5644723383298186No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC39110.4938817259972976No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT38080.48087486898432863No Hit
GTATAAGAGACAGCCAGGTCTGAAGGCAGTCTCTTATACACATCTCCGAG31330.39563575749157076No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA25290.3193625377261994No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG17830.22515753450605513No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA17260.21795956509111114No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA16210.20470014774779327No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA15800.19952266097564056No Hit
ATGCCCTGCAGTGCATCTGTCTCTTATACCTGTCTCTTATACACATCTCC13820.17451918827109825No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG12980.16391165439644395No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT11960.15103107754864945No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT11600.14648499160236902RNA PCR Primer, Index 17 (95% over 21bp)
GGACAAGGAGGAGTCCCTGTCTCTTATACCTGTCTCTTATACACATCTCC11450.14459078912475218No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC11030.139287022187425No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT10900.13764538004015708No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA10400.13133137178143428No Hit
GTGTATAAGAGACAGCTGACACACATCTCCGAGCCCACGAGACAGGCAGA10180.12855320814759624No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT10120.12779552715654952RNA PCR Primer, Index 17 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9560.12072383790677997No Hit
CCTTCAGACCTGGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCG9370.11832451476846532No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9030.1140309891525338No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG9020.11390470898735935No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGT8940.11289446766596371No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCC8930.11276818750078925No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG8920.1126419073356148No Hit
GAGATGTGTATAAGAGACAGCCAGGTCTGAAGGCAGTCTCTTATACACAT8230.10392857593857732No Hit
CCTTCAGACCTGGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAG8220.10380229577340289No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC8210.10367601560822841No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)