FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189747
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA1113459.35871239851834No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC873997.346015581463957No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC277812.335034255181984No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA146421.2306818172266876No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA143291.2043737029805497No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG118240.9938247375282308No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC90670.7620947983058582No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG54660.459425407250449No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC52780.4436237284061233No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC39560.33250766759655626No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG32940.27686558570855824No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA30270.2544238396902871No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC29750.25005316256313315No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA21660.18205551264260383No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT21400.1798701740790269No Hit
TCCTAGAACAGCTGGCTCAAGCAGAGCTTGCCTGATGCCAATACAGAGCT18160.15263749351752937No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17990.15120861830288287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17080.14355993333036352No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA16390.13776038098856314No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG15820.1329694464453367No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC14860.12490050405674484No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGC14520.12204275362745187No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA13570.11405786272207454No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA13550.11388975975564553No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT12320.10355142732026222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)