FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143721
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA676925.918576296142153No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC497834.352722385966508No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG205621.7978160757737245No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA98820.8640219074407133No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA82540.7216795005075539No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG68500.5989222896143378No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG62790.5489975264946608No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC59380.5191825628802829No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC46250.40438183787829374No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT45150.39476410768010733No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT44930.39284056164047No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT39020.34116712030294105No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA38460.3362708212929552No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT37260.3257787519858427No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC32630.285296851242567No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC26860.23484748465753447No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC26760.23397314554860846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25760.22522975445934804No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA25010.21867221114240273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23080.20179746634013013No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC23050.20153516460745236No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC22930.2004859576767411No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA21250.1857970606467836No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT20610.18020129034965696No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC19410.16970922104254446No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA18990.1660369967850551No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA15380.13447335495282503No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGG13610.11899755272483412No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG13140.11488815891288172No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC11900.10404635396219883No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACAC11810.10325944876416537No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)