FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004049208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007301
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC317153.1485127087136813No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC268092.6614686176227362No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA113921.1309429852645834No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC53690.5330085049056836No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA42420.421125363719484No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG37630.37357254683555363No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA35650.3539160588543047No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC27850.276481409231203No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT23530.23359452636302355No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC23150.22982206907369296No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT22080.2191996235484726No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA21350.21195253454528487No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT18750.18614098467091764No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC17300.17174608185636667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15860.15745045423364018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14540.1443461289128076No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT14480.14375047776186065No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA13690.135907737607726No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT11580.11496067213275873No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA10850.10771358312957101No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)