FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257421
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG1163619.253941201872722No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC633235.035942615878055No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG208661.6594283060327448No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG128271.0201038474782909No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA118700.9439956864089275No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG95140.7566280505892616No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC79480.6320874233848488No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG73120.5815077050566199No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC47860.38062033320582367No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC44210.35159266466839667No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA44060.35039974678329694No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC36250.2882884888991038No Hit
GTATCAACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTC31850.2532962309361781No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA29300.23301662688948252No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC28180.2241095066807378No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA27110.2156000257670263No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA23450.18649282937059267No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC23330.18553849506251288No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA22790.18124399067615382No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT22560.17941484991900086No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACA21810.17345026049350218No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT21230.1688376446711165No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT20400.16223683237356462No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGAT19720.15682893796111247No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG19170.15245490571574677No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19100.1518982107027002No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGA18950.15070529281760045No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC18800.14951237493250072No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGG18670.1484785127654143No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC18520.14728559488031456No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC18260.14521787054614166No Hit
GGTATCAACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT18190.14466117553309513No Hit
GAGGAATCGTGGTGGTGCTGGTGTTCACAGGGTTTGTGTGGGCAGCCCAC18150.14434306409706854No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC17360.13806036323554324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17040.13551547174733045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16610.13209577381004453No Hit
CGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC16180.12867607587275862No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG16100.12803985300070542No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG15710.12493826649944609No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC15670.12462015506341949No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14810.11778075918884764No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC13430.10680591464593005No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC13210.10505630174778376No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA12580.10004604663036486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)