FastQCFastQC Report
Thu 9 May 2024
EGAF00004149646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4927113
Total Bases320.2 Mbp
Sequences flagged as poor quality0
Sequence length65
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCTCGTAT1365652.7717042414087114TruSeq Adapter, Index 8 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCTCGTATG68970.13998055250610247TruSeq Adapter, Index 8 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)