FastQCFastQC Report
Thu 9 May 2024
EGAF00004149647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6700320
Total Bases435.5 Mbp
Sequences flagged as poor quality0
Sequence length65
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTAT3064734.5740054206366265TruSeq Adapter, Index 5 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATG126030.18809549394655778TruSeq Adapter, Index 5 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)