FastQCFastQC Report
Thu 9 May 2024
EGAF00004149648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9044640
Total Bases587.9 Mbp
Sequences flagged as poor quality0
Sequence length65
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTATCAATCTCGTAT3094333.4211754143890745TruSeq Adapter, Index 8 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTATCAATCTCGTATG101050.11172362858002088TruSeq Adapter, Index 8 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)