FastQCFastQC Report
Thu 9 May 2024
EGAF00004149656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6208581
Total Bases403.5 Mbp
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTAT2045463.294569242150501TruSeq Adapter, Index 23 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTATG80820.1301746727633899TruSeq Adapter, Index 23 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)