FastQCFastQC Report
Wed 8 May 2024
EGAF00004149659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8994493
Total Bases584.6 Mbp
Sequences flagged as poor quality0
Sequence length65
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTAT2282782.537975181035774TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTATG105070.11681592280965698TruSeq Adapter, Index 7 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)