FastQCFastQC Report
Wed 8 May 2024
EGAF00004149668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7630141
Total Bases495.9 Mbp
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATACAATCTCGTAT1044261.3685985619400742TruSeq Adapter, Index 22 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATACAATCTCGTATG123280.16156975342919613TruSeq Adapter, Index 22 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)