FastQCFastQC Report
Tue 7 Mar 2023
EGAF00004149925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109852396
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA50213934.571036393234427Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCACGCAT4131170.37606553433754875Illumina Paired End PCR Primer 2 (97% over 37bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2644320.24071573277291103Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCACGCA2300210.20939097222786113Illumina Paired End PCR Primer 2 (97% over 37bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG1760810.16028872051184026No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT1455140.1324632008936792No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCAT1371000.12480383222592616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)