FastQCFastQC Report
Sat 18 Mar 2023
EGAF00004149926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109852396
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA14248261.2970367983598647Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAG1981570.1803847774062206Illumina Single End PCR Primer 1 (100% over 57bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG1968480.17919317845374988Illumina Single End PCR Primer 1 (100% over 45bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGA1735670.158000195098157Illumina Single End PCR Primer 1 (100% over 57bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG1600680.14571188779532857No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCAT1384450.12602820242537086No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT1361350.12392538074454017No Hit
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG1253650.11412131602482299Illumina Single End PCR Primer 1 (100% over 45bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAGAA1251880.11396019072720089Illumina Single End PCR Primer 1 (100% over 57bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)