FastQCFastQC Report
Thu 9 Feb 2023
EGAF00004149929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127701319
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA18399501.4408230192203417Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGCTAT5580370.4369860893919193Illumina Paired End PCR Primer 2 (97% over 37bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG2128210.16665528724883413No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATG1628360.12751317000883913No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT1579260.1236682606230559No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCAT1346490.10544057105627859No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC1299080.10172800172878402Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)