FastQCFastQC Report
Mon 6 Feb 2023
EGAF00004149933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164027406
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5264250.3209372219176593Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGACCAAT2105100.1283383095139601Illumina Paired End PCR Primer 2 (97% over 37bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG1644000.100227153503848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)