FastQCFastQC Report
Sat 11 Mar 2023
EGAF00004149937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146267060
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1599741710.937128974903851Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCGTCCAT5696130.3894335471021295Illumina Paired End PCR Primer 2 (97% over 37bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5340050.36508903645154284Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCGTCCATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA3663030.25043437668057317Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCGTCCA2654740.18149951192018215Illumina Paired End PCR Primer 2 (97% over 37bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG1751700.11976038897616456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)