FastQCFastQC Report
Sat 25 May 2024
EGAF00004149939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138570369
Total Bases10.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAATCGATCTCGTA76088325.490951676689264Illumina Paired End Sequencing Primer 2 (100% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAATCGATCTCGTA4205040.3034588152103427Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAATCGATATCGTA3447510.24879128379891952Illumina Paired End Sequencing Primer 2 (100% over 36bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT2066780.14915021262590417No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1671370.12061525216837664No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAATCGAGCTCGTA1542320.11130229435991472Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG1426510.10294480777488585Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)