FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004149939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138570369
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAATCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA73906215.333478616918455Illumina Paired End PCR Primer 2 (97% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAATCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4047460.2920869756794831Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAATCGATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA3136740.22636441128333862Illumina Paired End PCR Primer 2 (97% over 36bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG2017850.1456191546982169No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT1633390.11787440646852865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)