FastQCFastQC Report
Wed 8 Feb 2023
EGAF00004149955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117566157
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA14503341.2336322263217296Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGCGAAT7359700.6260049820289695Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGCGAA1875830.1595552706549726Illumina Paired End PCR Primer 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph