FastQCFastQC Report
Fri 10 Feb 2023
EGAF00004149959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150464605
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCACTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA31875602.1184782959420922Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCACTTAT5759750.38279766859455083Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCACTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC2220120.14755098051133023Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCACTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA1735640.11535204575188962Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)