FastQCFastQC Report
Tue 7 Mar 2023
EGAF00004149961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110993712
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA37256353.356618075805952Illumina Paired End PCR Primer 2 (97% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2770560.24961414030373177Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGATGTAT2023510.18230852572981793Illumina Paired End PCR Primer 2 (97% over 37bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG1118920.10080931431503073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)