FastQCFastQC Report
Thu 9 Feb 2023
EGAF00004149963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161524112
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA28747671.7797757649953834Illumina Paired End PCR Primer 2 (97% over 42bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1950850.12077763349660142Illumina Paired End PCR Primer 2 (97% over 39bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG1775500.10992166915611956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)