FastQCFastQC Report
Fri 10 Feb 2023
EGAF00004149965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143544930
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG2473870.172341161753327No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT1863880.12984645295378944No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1725320.12019372610373631Illumina Paired End PCR Primer 2 (97% over 36bp)
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCAT1478770.1030179191978428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)