FastQCFastQC Report
Sat 11 Mar 2023
EGAF00004149971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127321872
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA118713559.323892912916016Illumina Paired End PCR Primer 2 (97% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6010340.47205872059436893Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCTTAT3030090.23798660453248754Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCTTATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA2308790.1813349084279879Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCTTA1311890.10303728490577016Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)