FastQCFastQC Report
Tue 14 Feb 2023
EGAF00004149975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146987102
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA9255450.6296776978431754Illumina Paired End PCR Primer 2 (97% over 40bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG1817690.12366323134937378No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1501000.10211780350632399No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT1497110.10185315443527827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)