FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004149977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131672056
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA96311847.314523895639634Illumina Paired End PCR Primer 2 (97% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6401940.4862033900344048Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATAT2131180.1618551471543818Illumina Paired End PCR Primer 2 (97% over 37bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG1988620.1510282485450064No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT1803450.1369652798616587No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCAT1393800.10585389507398593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)