FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004149995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004149995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114571199
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6213970.5423675456167654Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCCATGAT2241080.19560587822773853Illumina Paired End PCR Primer 2 (97% over 37bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG1186910.10359584348942703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)