FastQCFastQC Report
Fri 10 Feb 2023
EGAF00004150001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176591799
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA21406561.212205783123598Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCCATGAT3525580.19964573779555866Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCCATGA2366470.13400792185145585Illumina Paired End PCR Primer 2 (97% over 37bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG2311170.13087640610082918No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770850.10027928873412746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)