FastQCFastQC Report
Thu 23 Feb 2023
EGAF00004150002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176591799
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA4717250.26712735397185683Illumina Single End PCR Primer 1 (100% over 57bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG2049650.1160671113611567No Hit
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG2012420.11395885943718145Illumina Single End PCR Primer 1 (100% over 45bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)