FastQCFastQC Report
Thu 9 Feb 2023
EGAF00004150005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124249964
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA10172520.8187141205127431Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGGACAT4591570.3695429642136556Illumina Paired End PCR Primer 2 (97% over 38bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG1464250.11784711663980844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)