FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004150009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124942798
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA55253154.422275704118616Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCAT12881061.0309565822273326Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCA7736140.6191745441782087Illumina Paired End PCR Primer 2 (97% over 37bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCAT3059030.24483444015716693Illumina Paired End PCR Primer 2 (97% over 39bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2866760.22944579806832882Illumina Paired End PCR Primer 2 (97% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCA1915880.15334057109878393Illumina Paired End PCR Primer 2 (97% over 37bp)
GATGGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1445610.11570174697064171Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)