FastQCFastQC Report
Mon 6 Mar 2023
EGAF00004150026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132971493
Sequences flagged as poor quality0
Sequence length75
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA93524227.033403768731092Illumina Single End PCR Primer 1 (100% over 57bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG61322954.611736592293507Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG19776661.4872856996499242Illumina Single End PCR Primer 1 (100% over 45bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG11413880.8583704478673485Illumina Single End PCR Primer 1 (100% over 45bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAG10492130.7890510787902486Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAC7486890.5630447422290731Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAGAA6773650.509406177758717Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGA6509470.48953876151484593Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAACA6243430.46953146566535126Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAACAA4688250.35257557046456567Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAT4044010.30412608813830494Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAACAAA3487130.26224643503100326Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT3324550.2500197542340899Illumina Single End PCR Primer 1 (100% over 46bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAGAAA3271110.24600084771553254Illumina Single End PCR Primer 1 (100% over 57bp)
CGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT3070480.2309126513304622Illumina Single End PCR Primer 1 (100% over 46bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAGAG3036840.22838278577499313Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAATA2957860.22244316682223006Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAACAAAA2911990.21899355525774236Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAGGGG2855870.21477310178054482Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGG2478560.1863978469430286Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGAAAA2208670.16610101535071128Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAGGG2175440.1636019834717506Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAATAA1945080.1462779695193766Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAGAAG1883630.14165667824756994Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAGAGG1707090.12838014836759035Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGAAAAA1526370.11478926539540321Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAACAAAAA1497040.11258352946371747Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAGGAG1482730.11150735894948552Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAATAAA1452340.10922190668341222Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAACGGGG1432140.10770278408470603Illumina Single End PCR Primer 1 (100% over 57bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)