FastQCFastQC Report
Fri 10 Feb 2023
EGAF00004150029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157015765
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA16186081.030856997066505Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCAT3292080.2096655708425202Illumina Paired End PCR Primer 2 (97% over 38bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG2841590.18097482122256958No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT1800890.11469485245637595No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATG1628570.10372015829111174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)