FastQCFastQC Report
Fri 24 Feb 2023
EGAF00004150045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137211691
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA9089590.6624501114850337Illumina Paired End PCR Primer 2 (97% over 39bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG2922910.21302193557253077No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT2080290.15161171652640007No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCAT1888110.13760562137522234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)