FastQCFastQC Report
Tue 14 Feb 2023
EGAF00004150047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151568171
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATGTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA140420349.264500526301132Illumina Paired End PCR Primer 2 (97% over 41bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATGTCAAT8742950.5768328496884745Illumina Paired End PCR Primer 2 (97% over 42bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATGTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5778450.38124429171874086Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATGTCAA4080590.26922473056694735Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATGTCAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA3816690.25181342328132994Illumina Paired End PCR Primer 2 (97% over 41bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG2157410.1423392514250238No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT1696650.11193972908731609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)