FastQCFastQC Report
Mon 23 Jan 2023
EGAF00004150053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213664029
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAAACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA11323870.529984857675786Illumina Paired End PCR Primer 2 (97% over 36bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG4946630.23151440245470614No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT3004250.1406062599334397No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAAACCAT2895520.13551742956227789Illumina Paired End PCR Primer 2 (97% over 37bp)
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATG2446880.1145199784658184No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCAT2437400.11407629124133009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)