FastQCFastQC Report
Sat 11 Mar 2023
EGAF00004150055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131431631
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA42442413.22923862977855Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCTACAT6337760.48220964403918865Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCTACA3871050.2945295565874854Illumina Paired End PCR Primer 2 (97% over 37bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1673700.12734377465041122Illumina Paired End PCR Primer 2 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1319420.10038831519940586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)