FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004150056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131431631
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA9342120.7107969313718704Illumina Single End PCR Primer 1 (100% over 57bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG3314800.25220717225977357Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG1994610.15176027146768042Illumina Single End PCR Primer 1 (100% over 45bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)