FastQCFastQC Report
Fri 10 Feb 2023
EGAF00004150059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144738395
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA14142870.9771332617029502Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCTACAT4769760.32954351884308236Illumina Paired End PCR Primer 2 (97% over 37bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG2123230.14669431701242786No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATG1549040.1070234335540338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)