FastQCFastQC Report
Tue 14 Feb 2023
EGAF00004150068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154422926
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA13783500.8925811961366411Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAG1836230.11890915730997094Illumina Single End PCR Primer 1 (100% over 57bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG1736630.11245933780583849Illumina Single End PCR Primer 1 (100% over 45bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)