FastQCFastQC Report
Mon 27 Mar 2023
EGAF00004150073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61796936
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGGCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1576230.25506604405111605Illumina Paired End PCR Primer 2 (97% over 37bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG1169990.18932815698176364No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGGCCGAT1031490.16691604256884193Illumina Paired End PCR Primer 2 (97% over 38bp)
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATG772810.12505636201768966No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT756030.12234101703683171No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCAT634200.10262644736949418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)