FastQCFastQC Report
Thu 9 Feb 2023
EGAF00004150085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138514579
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAGTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA7651870.552423438402105Illumina Paired End PCR Primer 2 (97% over 36bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG2532070.18280169627487372No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAGTGGAT2469380.17827581889412522Illumina Paired End PCR Primer 2 (97% over 37bp)
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT1971880.1423590220059074No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCAT1443430.1042078032811261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)