FastQCFastQC Report
Tue 14 Feb 2023
EGAF00004150091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150258169
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA143699729.563521301793582Illumina Paired End PCR Primer 2 (97% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6187170.41176929288949343Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGAT5460440.363403869243209Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA4459980.2968211332323636Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGA2546140.16945101999745518Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2404780.16004321202662863Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1770160.11780790434096132Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)