FastQCFastQC Report
Fri 27 May 2022
EGAF00004150176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157283611
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA9126960.5802867788939561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA8558160.5441228075568534Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5438670.34578745779177206Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA3280620.2085799009281393Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2968340.18872532116521662No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2434520.15478535777004762No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2239130.1423625758439638Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2036370.1294712136282273No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1982040.12601694400314856Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA1963630.1248464469702441No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA1794040.11406401395502039No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA1711400.10880981108705597No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1701010.10814922096365145No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA1647720.10476107393032831No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1614940.10267694070172384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)