FastQCFastQC Report
Sat 25 May 2024
EGAF00004150196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196505739
Total Bases17.8 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5411480.27538534129021036Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3864250.196648200691991Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA3255060.16564707049090305Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3066790.15606617982795912Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCTGGCCACCACCATAAGCCAGGCCTCGGGGAGGGCACCCCCTAAGAC1997630.10165759077397733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)