FastQCFastQC Report
Sat 25 May 2024
EGAF00004150302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196671022
Total Bases17.8 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA39731802.0202162777188395Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA39210731.9937217797139428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA27178311.381917362487698Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA9342920.47505320839793064Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAA7994780.4065052349196619Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA7367290.3745996703062844Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA6867630.34919379226086494Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT6152120.3128127335403789Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC5864270.29817661699037695Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA5476860.2784782396666449Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAA4607660.23428260824311983No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT4465470.22705276835343846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4373480.2223754143098926No Hit
GTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAA4053140.20608730044632603No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAA3877650.1971642777144871Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAA3680010.18711500873778955Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAA3487360.17731946295575768Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA3362490.17097028152932464Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAA3126350.1589634287861686Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGAAAAAAAAAAAAAAA2911440.14803604366280254Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2437600.12394301789920022Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAA2414510.12276897610264109Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAA2193720.11154261454948865Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCA2129160.10825997538162993Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA2058570.1046707328342454Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)